Table 3 RNA structure webserver prediction of free energy of folding of the predicted siRNA’s
Alias | Target sequence (21nt target + 2nt overhang) | Target position in mRNA of cell surface binding protein | RNA oligo sequences 21nt guide (5′ → 3′) 21nt passenger (5′ → 3′) | Free energy of folding |
|---|---|---|---|---|
S1 | GCGAATATCGTTGACTCATAAGA | 18–40 | UUAUGAGUCAACGAUAUUCGC GAAUAUCGUUGACUCAUAAGA | − 0.1 |
S2 | GAGAATAGCGGTGAGTATAAATA | 43–65 | UUUAUACUCACCGCUAUUCUC GAAUAGCGGUGAGUAUAAAUA | 1.8 |
S3 | TACGAATACTATGGCAATAATTG | 64–86 | AUUAUUGCCAUAGUAUUCGUA CGAAUACUAUGGCAAUAAUUG | 1.5 |
S4 | GACGACAATAGTGTTCTGAATTT | 302–324 | AUUCAGAACACUAUUGUCGUC CGACAAUAGUGUUCUGAAUUU | – |
S6 | AGGTAGCAAATTGTCTAGATAAA | 433–455 | UAUCUAGACAAUUUGCUACCU GUAGCAAAUUGUCUAGAUAAA | 1.7 |
S8 | ATCAAGTGATTGGATCCATAATC | 671–693 | UUAUGGAUCCAAUCACUUGAU CAAGUGAUUGGAUCCAUAAUC | 1.5 |
S9 | GTCTTCCTTTCCCCAATATATAT | 694–716 | AUAUAUUGGGGAAAGGAAGAC CUUCCUUUCCCCAAUAUAUAU | 1.8 |
S10 | TGGTTGGTTTCGACTCATTATAA | 813–835 | AUAAUGAGUCGAAACCAACCA GUUGGUUUCGACUCAUUAUAA | – |