Table 4 Calculation of free energy of binding, heat capacity, concentration plot and conservancy search against other strains of monkeypox virus
Alias | Target position in mRNA of Spike | Target sequence (21nt target + 2nt overhang) | RNA oligo sequences 21nt guide (5′ → 3′) 21nt passenger (5′ → 3′) | Free energy of binding | Tm (°C) | Validity | % of RNA sequence matched with other strains | |
|---|---|---|---|---|---|---|---|---|
Tm (Cp) | Tm (Conc) | |||||||
S2 | 43–65 | GAGAATAGCGGTGAGTATAAATA | UUUAUACUCACCGCUAUUCUC GAAUAGCGGUGAGUAUAAAUA | − 33.9 | 89.8 | 88.7 | 74.6 | 98.80% (82/83) |
S3 | 64–86 | TACGAATACTATGGCAATAATTG | AUUAUUGCCAUAGUAUUCGUA CGAAUACUAUGGCAAUAAUUG | − 29.5 | 83.6 | 82.2 | 68.9 | 100.00% (83/83) |
S6 | 433–455 | AGGTAGCAAATTGTCTAGATAAA | UAUCUAGACAAUUUGCUACCU GUAGCAAAUUGUCUAGAUAAA | − 33.0 | 83.6 | 82.2 | 84.6 | 100.00% (83/83) |
S8 | 671–693 | ATCAAGTGATTGGATCCATAATC | UUAUGGAUCCAAUCACUUGAU CAAGUGAUUGGAUCCAUAAUC | − 32.8 | 87.3 | 86.1 | 97.7 | 98.80% (82/83) |
S9 | 694–716 | GTCTTCCTTTCCCCAATATATAT | AUAUAUUGGGGAAAGGAAGAC CUUCCUUUCCCCAAUAUAUAU | − 34.5 | 92.9 | 92.7 | 70.6 | 100.00% (83/83) |