Fig. 5
Sections of heart from mice of all studied groups. Sections stained with H&E appeared in the 1st raw (X200); Control group (A), AB-PINACA group (B), and MDMB-4en-PINACA group (C)and 2nd raw (X400); Control group (A1), AB-PINACA group (B1), and MDMB-4en-PINACA group (C1): stained sections appeared as following, normal cardiac muscle cells with oval vesicular nuclei (thin long arrows), normal capillaries (curved arrows), necrotic myocytes with pyknotic nuclei (thick short arrows), infarcted myocytes (arrowheads), intracellular vacuolations (tailed arrows), edema (e), inflammatory cell infiltrations (circle), degenerated and congested blood vessels (zigzagged arrows) and thrombus (th). Sections stained with PAS stain in the 3rd raw (X400); the control group (A2) showed cardiomyocytes with a significant positive reaction of pink color. AB-PINACA group (B2) showed moderate reaction within the degenerated myocytes. MDMB-4en-PINACA group (C2) showed mild reaction within the degenerated myocytes. Sections stained with Bromophenol Blue stain in the 4th raw (X400); cardiomyocytes appeared with a significant blue color positive reaction in the control group (A3), a blue color moderate reaction in AB-PINACA group (B3) and a blue color mild reaction in MDMB-4en-PINACA group (C3)