Molecular approach for identification and validation of Eurycoma longifolia, Labisia pumila, and Orthosiphon stamineus in herbal products

Azli, Siti Nor Amira Mohd; Bakar, Adibah Abu; Shahimi, Salwa; Nelson, Bryan Raveen; Jamaludin, Azi Azeyanty; Al-Obaidi, Jameel R.

doi:10.1186/s43088-024-00481-1

Beni-Suef University Journal of Basic and Applied Sciences

Table 4 Identification results of herbal product based on ITS2 GenBank BLAST

From: Molecular approach for identification and validation of Eurycoma longifolia, Labisia pumila, and Orthosiphon stamineus in herbal products

No.	Code	Common name claim species	Species identification	Percentage identification (%)	Reference GenBank accession
1	HMP01	Tongkat Ali	Orthosiphon stamineus	100.00	MT251295.1
2	HMP02	Tongkat Ali	Orthosiphon stamineus	100.00	MT251295.1
3	HMP05	Tongkat Ali (Eurycoma longifolia)	Pedicularis lachnoglossa	98.78	MZ198677.1
4	HMP13	Kacip fatimah (Labisia pumila)	Orthosiphon stamineus	100	MT251295.1
5	HMP14	Tongkat Ali, Misai Kucing	Orthosiphon stamineus	100.00	MT251295.1
6	HMP15	Tongkat Ali	Orthosiphon stamineus	100.00	MT251295.1
7	HMP16	Tongkat Ali	Orthosiphon stamineus	100.00	MT251295.1
8	HMP17	Eurycoma longifolia	Orthosiphon stamineus	100.00	MT251295.1
9	HMP18	Eurycoma longifolia	Coriandrum sativum	100.00	ON685496.1
10	HMP19	Labisia Pathoina	Coriandrum sativum	93.13	ON685496.1
11	HMP21	Kacip Fatimah	Orthosiphon stamineus	99.29	MT251295.1
12	HMP22	Tongkat Ali	Orthosiphon stamineus	100.00	MT251295.1
13	HMP24	Misai Kucing	Orthosiphon stamineus	99	MT251295.1
14	HMP26	Tongkat Ali	Orthosiphon stamineus	99.64	MT251295.1
15	HMP27	Tongkat Ali	Orthosiphon stamineus	99.64	MT251295.1
16	HMP32	Kacip Fatimah	Orthosiphon stamineus	100.00	MT251295.1
17	HMP33	Labisia pumila	Orthosiphon stamineus	96.09	MT251295.1
18	HMP38	Tongkat Ali, Misai Kucing	Orthosiphon stamineus	100.00	MT251295.1
19	HMP39	Kacip Fatimah	Orthosiphon stamineus	100.00	MT251295.1
20	HMP40	Tongkat Ali	Orthosiphon stamineus	99.64	MT251295.1
21	HMP41	Tongkat Ali	Orthosiphon stamineus	100	MT251295.1
22	HMP42	Kacip fatimah (Labisia pumila)	Orthosiphon stamineus	99.64	MT251295.1
23	HMP43	Tongkat Ali (Eurycoma longifolia)	Orthosiphon stamineus	100	MT251295.1
24	HMP44	Tongkat Ali	Orthosiphon stamineus	100	MT251295.1
25	HMP45	Tongkat Ali	Orthosiphon stamineus	100	MT251295.1
26	HMP46	Kacip Fatimah	Coriandrum sativum	99.33	ON685496.1
27	HMP52	Misai Kucing	Orthosiphon stamineus	100	MT251295.1
28	HMP53	Kacip Fatimah (Labisia pumila)	Orthosiphon stamineus	93.55	MT251295.1
29	HMP60	Tongkat Ali	Coriandrum sativum	87.34	ON685496.1
30	HMP61	Tongkat Ali (Eurycoma longifolia)	Orthosiphon stamineus	96.35	MT251295.1
31	HMP67	Labisia Pathoina	Coriandrum sativum	89.35	ON685496.1
32	HMP78	Tongkat ali, Misai Kucing	Orthosiphon stamineus	89.96	MT251295.1
33	HMP79	Kacip Fatimah	Orthosiphon stamineus	100	MT251295.1
34	HMP87	Tongkat Ali	Coriandrum sativum	82.83	ON685496.1
35	HMP88	Tongkat Ali	Coriandrum sativum	86.54	ON685496.1
36	HMP92	Tongkat Ali	Anethum foeniculum	96.01	ON685481.1
37	HMP98	Tongkat ali (Eurycoma longifolia), Misai Kucing (Orthosiphon stamineus)	Anethum foeniculum	98.67	ON685481.1
38	HMP101	Kacip Fatimah	Hordeum vulgare	79.31	XR_006625164.1
39	HMP109	Kacip Fatimah	Deverra tortuosa	85.27	KJ473888.1
40	HMP116	Kacip Fatimah (Labisia pumila)	Orthosiphon stamineus	92.65	MT251295.1
41	HMP118	Tongkat Ali (Eurycoma longifolia)	Anethum graveolens	99.32	ON685466.1
42	HMP124	Kacip Fatimah	Anethum foeniculum	91.83	MZ779166.1
43	HMP125	Tongkat Ali	Anethum foeniculum	93.82	KP406140.1
44	HMP127	Kacip Fatimah	Anethum graveolens	85.71	MN257763.1
45	HMP135	Tongkat Ali	Orthosiphon stamineus	100	MT251295.1
46	HMP136	Tongkat Ali	Orthosiphon stamineus	89.29	MT251295.1
47	HMP137	Tongkat Ali	Orthosiphon stamineus	100	MT251295.1
48	HMP139	Tongkat Ali	Salacia menglaensis	97.24	MZ568402.1
49	HMP140	Kacip Fatimah	Orthosiphon aristatus	93.93	MW315930.1
50	HMP142	Kacip Fatimah	Orthosiphon aristatus	93.93	MW315930.1

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