Table 6 The fragment size (bps) by in silico restrictions predictions and restriction profiles exhibited by these enzymes MboI, TaqI, MspI, HinfI, EcoRI, EcoRV, and BamHI on plant species specimen
Plant species | E. longifolia | O. stamineus | L. pumila | |||
|---|---|---|---|---|---|---|
Amplified fragment | 315 | 316 | 315 | |||
Restriction enzyme | In silico restriction | Restriction profiles | In silico restriction | Restriction profiles | In silico restriction | Restriction profiles |
EcoRI | NA | NA | NA | NA | NA | NA |
EcoRV | NA | NA | NA | NA | NA | NA |
BamHI | NA | NA | NA | NA | NA | NA |
HinfI | 21 + 45 + 46 + 203 | 50 + 265 | 31 + 45 + 45 + 59 + 136 | 76 + 104 + 136 | 21 + 81 + 98 + 115 | 102 + 213 |
Mspl | 6 + 66 + 243 | 66 + 249 | 26 + 65 + 225 | 91 + 225 | 73 + 242 | 73 + 242 |
TaqI | 19 + 26 + 51 + 219 | 41 + 51 + 219 | 39 + 108 + 169 | 39 + 108 + 169 | 33 + 54 + 55 + 173 | 109 + 206 |
Mbol | 46 + 269 | 46 + 269 | 20 + 296 | 20 + 296 | 30 + 51 + 58 + 269 | 51 + 88 + 269 |