Table 1 The chronic unpredictable stress protocol
S. No. | Stressors | Procedure |
|---|---|---|
1 | Restraint stress | Restrained in Plexiglas restrainer for 2 h |
2 | Noise stress | Exposed to 100 dB noise for 4 h |
3 | Food deprivation | Deprived of food for 24 h |
4 | Cage rotation stress | Animal cage was placed in rotator for 1 h at 50 rpm |
5 | Forced swimming stress | Rats were forced to swim in a cylindrical tank which is 60cm long and 30 cm width with water to a 30 cm depth at 8 °C for 5 min |
6 | Sleep deprivation | Rats were placed in sleep deprivation tank for 24 h, six platforms were placed at the bottom of the tank (110 × 60 × 40 cm). The tank was filled with water at room temperature and a depth of 1.0cm below the platform surface, food and water was placed on the lid hanging over tank |
7 | Water deprivation | Deprived of water for 24 h |