Table 2 Primer sequences of forward and reverse primers
Gene | Forward primer | Reverse primer | Tm (°C) | Amplicon size (bp) |
|---|---|---|---|---|
GAPDH | CGGCAAGTTCAACGGCACAG | CGCCAGTAGACTCCACGACAT | 60 | 143bp |
TrkB | ACAAAGGCCTTAACAAACCT | CCACATCAAAGGCAGGAATA | 60 | 98bp |
BDNF | CCTCTGCTCTTTCTGCTGGA | GCTGTGACCCACTCGCTAAT | 60 | 137bp |
IL-1β | CCTATGTCTTGCCCGTGGAG | CACACACTAGCAGGTCGTCA | 60 | 637bp |
TNF-α | TCCCAGGTTCTCTTCAAGGGA | GGTGAGGAGCACGTAGTC GG | 60 | 51bp |
ERK | GCGTTGGTACAGAGCTCCAGAA | TGCAGCCCACAGACCAAATATC | 57 | 289bp |
AKT | TCACCTCTGAGACCGACACC | ACTGGCTGAGTAGGAGAACTGG | 59 | 588bp |