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Beni-Suef University Journal of Basic and Applied Sciences
Fig. 4 | Beni-Suef University Journal of Basic and Applied Sciences

Fig. 4

From: Cloning, heterologous expression and purification of the novel thermo-alkalistable cellulase from Geobacillus sp. TP-3 and its molecular characterisation

Fig. 4

Confirmation of pET-Cel3 construct in the transformed E. coli DH5α host. A Colony PCR analysis of cellulase gene from two clones. L1 and L2:1200 bp PCR product amplified using cellulase primers from two random colonies; M Marker; B Restriction digestion of pET-Cel3 recombinant expression vector. L1, Fragments ~ 5300 corresponding to pET 28 ( +) vector and ~ 1200 bp fragment of cellulase gene resulted double digestion of expression with XhoI and BamHI enzymes; M- 100 bp DNA Marker. L denotes lane here

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