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Beni-Suef University Journal of Basic and Applied Sciences
Fig. 5 | Beni-Suef University Journal of Basic and Applied Sciences

Fig. 5

From: Cloning, heterologous expression and purification of the novel thermo-alkalistable cellulase from Geobacillus sp. TP-3 and its molecular characterisation

Fig. 5

Protein purification of rCel_TP. A Zymogram analysis of purified rCel_TP cellulase enzyme; B A SDS-PAGE (12% w/v) analysis of His-tagged rCel_TP cellulase enzyme purified using immobilised metal affinity chromatography (Ni–NTA). M- Protein molecular weight marker ((14.3–97.4 kDa); L1 Cell-free extract of uninduced cells; L2 Cell-free extract of IPTG (0.5 mM) induced cells; L3 Proteins eluted with buffer containing 100 mM imidazole; L4-6 Eluted purified rCel_TP proteins at 200 mM, 250 mM and 500 mM imidazole, respectively. L denotes lane here

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