Table 1 Degenerate PCR oligo-primers designed for thermophilic cellulase genes based on literature
Forward primer | 5’ATCCTCGAGATGGCRAARTTNGAYGMAACRTTRAC3’ Xho1 |
Reverse primer | 5’TGCGGATCCTTATTMGTCAAABGTMAKTTSBTTCAC3’ BamH1 |
R | A, G |
N | A, C, G, T |
Y | C, T |
M | A, C |
B | C, G, T |
K | G, T |
S | C, G |