Table 2 Effect of metal ions, inhibitors, detergents and solvents on rCel_TP enzyme

Metal ions

MgCl₂

82.16 ± 1.23

80.11 ± 2.12

75.11 ± 0.54

MnCl₂

90.03 ± 0.23

85.05 ± 2.12

80.52 ± 1.23

CaCl₂

79.53 ± 1.25

78.22 ± 2.32

70.45 ± 1.98

HgCl₂

133.85 ± 1.30

130.23 ± 1.74

125.42 ± 2.45

KCl

114.70 ± 2.25

105.77 ± 3.32

103.68 ± 2.10

NH₄Cl₂

62.74 ± 2.21

54.69 ± 0.87

50.65 ± 1.63

CuCl₂

132.80 ± 1.85

125.96 ± 1.74

120.93 ± 1.41

CoCl₂

113.64 ± 0.98

110.05 ± 0.64

98.98 ± 0.73

NaCl

93.44 ± 0.54

92.66 ± 0.74

85.36 ± 3.63

FeCl₃

73.76 ± 1.45

66.41 ± 2.74

52.32 ± 1.09

Inhibitors

EDTA

79.49 ± 1.63

65.82 ± 2.23

52.06 ± 2.14

β-mercaptoethanol

57.28 ± 3.54

37.91 ± 2.74

27.94 ± 1.94

DTT

27.37 ± 1.13

21.67 ± 1.20

20.01 ± 0.87

Detergents

0.5%

1%

10%

SDS

64.37 ± 1.2

56.92 ± 0.56

52.77 ± 1.32

Tween 20

74.87 ± 2.32

70.17 ± 1.47

66.58 ± 1.54

Tween 80

63.82 ± 2.05

61.06 ± 1.04

55.54 ± 1.45

Triton × 100

48.63 ± 1.45

44.21 ± 2.12

30.96 ± 2.36

Solvent

0.5%

1%

10%

Chloroform

85.80 ± 1.03

70.60 ± 1.36

55.13 ± 1.45

Acetone

81.73 ± 0.96

51.87 ± 1.87

47.80 ± 1.50

Isopropanol

68.16 ± 2.30

64.08 ± 2.10

52.14 ± 3.63

DMF

50.24 ± 1.50

25.81 ± 1.33

24.08 ± 1.20

Glycerol

80.88 ± 3.32

78.01 ± 3.30

60.02 ± 2.97

Methanol

68.16 ± 0.90

60.08 ± 1.15

41.56 ± 1.10

Ethanol

78.64 ± 2.20

69.97 ± 2.42

67.35 ± 2.60