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Beni-Suef University Journal of Basic and Applied Sciences
Fig. 2 | Beni-Suef University Journal of Basic and Applied Sciences

Fig. 2

From: The effect of cigarette and e-cigarette smoke on dental pulp stem cells proliferation capacity and differentiation [in vitro study]

Fig. 2

A Linear regression curves were determined using the MTT assay of the DPSCs treated with the log dose of TE-p. B e-Cig-LV versus the normalized response in DPSCs cells after treatment for 21 days. There was a high linear regression coefficient between the response of the control untreated DPSCs and the log dose of TE-p and e-Cig-LV. IC50 half maximum cytotoxic effect, CI confidence interval. C The Bar chart showed a mild significant difference between the calculated IC50 of TE-p and e-Cig-LV on DPSCs after 14 days of exposure (p = 0.036), t = 3.09, and the dF = 4. D The bar chart showed a mild significant difference between the calculated IC50 of TE-p and e-Cig-LV on DPSCs after 21 days of exposure (p = 0.027), t = 3.4. Data are presented as mean and SD. E DPSCs were cultured for 14 and 21 days in DMEM media treated with TE-p and e-Cig-LV smoke extracts. The cell viability was compared to untreated cells co-cultured in DMEM. Images for DPSCs cultured in media treated with smoke extracts showed few scattered colonies, in contrast with dense colonies of cells observed in control untreated cells. The magnification [10X] (Scale bar = 50 μm)

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