Table 1 Primers used for amplification of DNA extracted from the identified sarcocysts using 18S and 28S rRNA regions, with expected amplicon size and melting temperature (MT)
Gene | Nucleotide sequences | bp | Melting temperature (°C) | Reference |
|---|---|---|---|---|
Sarcocystis 18S rRNA fragment | 1L (F) (5′-CCATGCATGTCTAAGTATAAGC-3′) | 1391 | 51.2 | Yang et al. [25] |
1H (R) (5′-TATCCCCATCACGATGCATAC-3′) | 54.3 | |||
18S1F (5′-GGATAACCGTGGTAATTCTATG-3′) | 1085 | 50.6 | Rosenthal et al. [26] | |
18S11R (5′-TCCCATGTCTGGACCTGGTGAG-3′) | 54.5 | |||
Sarcocystis 28S rRNA fragment | KL5a (5′-GAC CCT GTT GAG CTT GAC-3′) | 645 | 53.2 | Mugridge et al. [27] |
KL2 (5′-ACT TAG AGG CGT TCA GTC-3′) | 51.3 | |||
Wf (5′-CTCTCTTAAGGTAGCCAAATGC-3′) | 812 | 53.4 | This study | |
Wr (5′-CTTTTCAACTAGCCGCATTTAC-3′) | 52.4 |