No. of steps | Bacteriocin | Source | Purification method | Yield (%) | Purification fold | References |
---|---|---|---|---|---|---|
1 | Bacteriocin-like inhibitory substance (BLIS) | Pediococcus acidilactici Kp10 isolated from dried curd | ATPS (PEG/Na-citrate) | 81.18 | 8.43 | Abbasiliasi et al. [1] |
1 | BLIS | Pediococcus acidilactici Kp10 | AMTPS (Triton X-100 (TX-100)) | 64.3 | 5.8 | Jamaluddin et al. [69] |
1 | BLIS | Lactococcus lactis Gh1 | ATPS (PEG2000/Dextran T500) | 77.24 | 2.92 | Jawan et al. [70] |
1 | BLIS | Lactobacillus bulgaricus FTDC 1211 | PEG 4000 impregnated Amberlite XAD4 resins/sodium citrate | 82.69 | 3.26 | Abdul Aziz et al. [2] |
1 | Nisin | Lactococcus lactis ATCC 11,454 | ATPS (PEG/ MgSO4) | – | – | C. Li et al. [84] |
1 | Nisin | Lactococcus lactis ATCC 11,454 | ATPS (PEG 4000 and Na2SO4) | 111.60 | – | C. Li et al. [83] |
1 | Not specified | Lactobacillus plantarum ST16Pa isolated from a papaya species | ATPS (PEG/ sodium polyacrylate (NaPA)) | 93.36 | – | da Silva Sabo et al. [37] |
2 | Bacteriocin LAB-3H | Latilactobacillus curvatus LAB-3H | Ammonium sulphate precipitation, cation exchange chromatography | 5.21 | 249.22 | Heidari et al. [64] |
2 | Pediocin PA-1 | Pediococcus acidilactici PAC 1.0 cultivated in MRS broth | Ion exchange chromatography, hydrophobic interaction chromatography | 73 | – | Beaulieu et al. [14] |
2 | Pediocin PD-1 | Pediococcuc damnosus NCFB 1832 isolated from beer | Ammonium sulphate precipitation, cation exchange chromatography | 34 | 1700 | Bauer et al. [13] |
2 | Pediocin NCDC35 | Pediococcus pentosaceus NCDC35 | Ammonium sulphate precipitation, ion-exchange chromatography | 14.4 | 14.01 | Meena et al. [92] |
2 | Paracasin SD1 | Lactobacillus paracasei SD1 | Ammonium sulfate precipitation, gel filtration chromatography | – | 871 | P Wannun et al. [150] |
3 | Enterocin TJUQ1 | Enterococcus faecium TJUQ1 isolated from pickled Chinese celery | Ammonium sulfate precipitation, reversed-phase chromatography (Sep-Pak C 8) and cation-exchange chromatography | – | 35.89 | Qiao et al. [118] |
3 | Enterocin LD3 | Enterococcus hirae LD3 | Ammonium sulphate precipitation, ion-exchange chromatography, gel filtration chromatography | 0.37 | 13.2 | Gupta et al. [57] |
3 | Plantaricin GZ1-27 | Lactobacillus plantarum GZ1-27 | Ammonium sulphate precipitation, gel-filtration chromatography, and RP-HPLC | 0.1 | 10.55 | Pei et al. [110] |
3 | Bacteriocin LF-BZ532 | Lactobacillus fermentum BZ532 | Ammonium sulphate precipitation, cation-exchange chromatography, and RP-HPLC | 0.85 | 4.2 | Rasheed et al. [121] |
3 | Fermencin SD11 | Lactobacillus fermentum SD11 | Ammonium sulfate precipitation, gel filtration chromatography, and reverse-phase high-performance liquid chromatography | – | 7,526 | Phirawat Wannun et al. [151] |
3 | Plantaricin KL-1X | Lactobacillus plantarum KL-1 | Ammonium sulphate precipitation, ion-exchange chromatography, RP-HPLC | 0.004 | 0.04 | Rumjuankiat et al. [127] |
 | KL-1Y |  |  | 0.8 | 10.5 |  |
 | KL-1Z |  |  | 0.1 | 0.9 |  |
3 | Sakacin A | Lactobacillus sakei 2675 | Ion exchange chromatography, hydrophobic interaction chromatography, RP-HPLC | 10 | – | Guyonnet et al. [58] |
 | Sakacin P | Lactobacillus sakei 2525 |  | 50 | – |  |
 | Enterocin A | Enterococcus faecalis 336 |  | 66 | – |  |
 | Pediocin PA-1 | Pediococcus acidilactici 1521 |  | 25 | – |  |
4 | Bacteriocin-zrx01 | Lactobacillus rhamnosus zrx01 isolated from Kefir | Ammonium sulphate precipitation, ion-exchange chromatography, gel filtration chromatography, RP-FPLC | 1.76 | 8.5 | Pei et al. [110] |
4 | Paracin 54 | Lactobacillus paracasei ZFM54 | Ammonium sulphate, strong cation-exchange chromatography, G-25 gel column, and RP-HPLC | 1.93 | 45.64 | Zhu et al. [161] |
4 | Enterocin HDX-2 | Enterococcus faecium HDX-2 isolated from pickled Chinese cucumber | Ammonium sulphate precipitation, ion-exchange chromatography, hydrophobic interaction chromatography, RP-HPLC | 6.38 | 56.61 | Du et al. [43] |
4 | Plantaricin ZJ5 | Lactobacillus plantarum ZJ5 | Ammonium sulphate precipitation, ion-exchange chromatography, hydrophobic interaction chromatography, RP-HPLC | 1.7 | 139.5 | Song et al. [138] |