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Table 1 Summary of purification methods for purifying bacteriocin produced by lactic acid bacteria

From: Bacteriocins from lactic acid bacteria: purification strategies and applications in food and medical industries: a review

No. of steps

Bacteriocin

Source

Purification method

Yield (%)

Purification fold

References

1

Bacteriocin-like inhibitory substance (BLIS)

Pediococcus acidilactici Kp10 isolated from dried curd

ATPS (PEG/Na-citrate)

81.18

8.43

Abbasiliasi et al. [1]

1

BLIS

Pediococcus acidilactici Kp10

AMTPS (Triton X-100 (TX-100))

64.3

5.8

Jamaluddin et al. [69]

1

BLIS

Lactococcus lactis Gh1

ATPS (PEG2000/Dextran T500)

77.24

2.92

Jawan et al. [70]

1

BLIS

Lactobacillus bulgaricus FTDC 1211

PEG 4000 impregnated Amberlite XAD4 resins/sodium citrate

82.69

3.26

Abdul Aziz et al. [2]

1

Nisin

Lactococcus lactis ATCC 11,454

ATPS (PEG/ MgSO4)

C. Li et al. [84]

1

Nisin

Lactococcus lactis ATCC 11,454

ATPS (PEG 4000 and Na2SO4)

111.60

C. Li et al. [83]

1

Not specified

Lactobacillus plantarum ST16Pa isolated from a papaya species

ATPS (PEG/ sodium polyacrylate (NaPA))

93.36

da Silva Sabo et al. [37]

2

Bacteriocin LAB-3H

Latilactobacillus curvatus LAB-3H

Ammonium sulphate precipitation, cation exchange chromatography

5.21

249.22

Heidari et al. [64]

2

Pediocin PA-1

Pediococcus acidilactici PAC 1.0 cultivated in MRS broth

Ion exchange chromatography, hydrophobic interaction chromatography

73

Beaulieu et al. [14]

2

Pediocin PD-1

Pediococcuc damnosus NCFB 1832 isolated from beer

Ammonium sulphate precipitation, cation exchange chromatography

34

1700

Bauer et al. [13]

2

Pediocin NCDC35

Pediococcus pentosaceus NCDC35

Ammonium sulphate precipitation, ion-exchange chromatography

14.4

14.01

Meena et al. [92]

2

Paracasin SD1

Lactobacillus paracasei SD1

Ammonium sulfate precipitation, gel filtration chromatography

871

P Wannun et al. [150]

3

Enterocin TJUQ1

Enterococcus faecium TJUQ1 isolated from pickled Chinese celery

Ammonium sulfate precipitation, reversed-phase chromatography (Sep-Pak C 8) and cation-exchange chromatography

35.89

Qiao et al. [118]

3

Enterocin LD3

Enterococcus hirae LD3

Ammonium sulphate precipitation, ion-exchange chromatography, gel filtration chromatography

0.37

13.2

Gupta et al. [57]

3

Plantaricin GZ1-27

Lactobacillus plantarum GZ1-27

Ammonium sulphate precipitation, gel-filtration chromatography, and RP-HPLC

0.1

10.55

Pei et al. [110]

3

Bacteriocin LF-BZ532

Lactobacillus fermentum BZ532

Ammonium sulphate precipitation, cation-exchange chromatography, and RP-HPLC

0.85

4.2

Rasheed et al. [121]

3

Fermencin SD11

Lactobacillus fermentum SD11

Ammonium sulfate precipitation, gel filtration chromatography, and reverse-phase high-performance liquid chromatography

7,526

Phirawat Wannun et al. [151]

3

Plantaricin KL-1X

Lactobacillus plantarum KL-1

Ammonium sulphate precipitation, ion-exchange chromatography, RP-HPLC

0.004

0.04

Rumjuankiat et al. [127]

 

KL-1Y

  

0.8

10.5

 
 

KL-1Z

  

0.1

0.9

 

3

Sakacin A

Lactobacillus sakei 2675

Ion exchange chromatography, hydrophobic interaction chromatography, RP-HPLC

10

Guyonnet et al. [58]

 

Sakacin P

Lactobacillus sakei 2525

 

50

 
 

Enterocin A

Enterococcus faecalis 336

 

66

 
 

Pediocin PA-1

Pediococcus acidilactici 1521

 

25

 

4

Bacteriocin-zrx01

Lactobacillus rhamnosus zrx01 isolated from Kefir

Ammonium sulphate precipitation, ion-exchange chromatography, gel filtration chromatography, RP-FPLC

1.76

8.5

Pei et al. [110]

4

Paracin 54

Lactobacillus paracasei ZFM54

Ammonium sulphate, strong cation-exchange chromatography, G-25 gel column, and RP-HPLC

1.93

45.64

Zhu et al. [161]

4

Enterocin HDX-2

Enterococcus faecium HDX-2 isolated from pickled Chinese cucumber

Ammonium sulphate precipitation, ion-exchange chromatography, hydrophobic interaction chromatography, RP-HPLC

6.38

56.61

Du et al. [43]

4

Plantaricin ZJ5

Lactobacillus plantarum ZJ5

Ammonium sulphate precipitation, ion-exchange chromatography, hydrophobic interaction chromatography, RP-HPLC

1.7

139.5

Song et al. [138]