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Beni-Suef University Journal of Basic and Applied Sciences

Table 1 Summary of purification methods for purifying bacteriocin produced by lactic acid bacteria

From: Bacteriocins from lactic acid bacteria: purification strategies and applications in food and medical industries: a review

No. of steps Bacteriocin Source Purification method Yield (%) Purification fold References
1 Bacteriocin-like inhibitory substance (BLIS) Pediococcus acidilactici Kp10 isolated from dried curd ATPS (PEG/Na-citrate) 81.18 8.43 Abbasiliasi et al. [1]
1 BLIS Pediococcus acidilactici Kp10 AMTPS (Triton X-100 (TX-100)) 64.3 5.8 Jamaluddin et al. [69]
1 BLIS Lactococcus lactis Gh1 ATPS (PEG2000/Dextran T500) 77.24 2.92 Jawan et al. [70]
1 BLIS Lactobacillus bulgaricus FTDC 1211 PEG 4000 impregnated Amberlite XAD4 resins/sodium citrate 82.69 3.26 Abdul Aziz et al. [2]
1 Nisin Lactococcus lactis ATCC 11,454 ATPS (PEG/ MgSO4) – – C. Li et al. [84]
1 Nisin Lactococcus lactis ATCC 11,454 ATPS (PEG 4000 and Na2SO4) 111.60 – C. Li et al. [83]
1 Not specified Lactobacillus plantarum ST16Pa isolated from a papaya species ATPS (PEG/ sodium polyacrylate (NaPA)) 93.36 – da Silva Sabo et al. [37]
2 Bacteriocin LAB-3H Latilactobacillus curvatus LAB-3H Ammonium sulphate precipitation, cation exchange chromatography 5.21 249.22 Heidari et al. [64]
2 Pediocin PA-1 Pediococcus acidilactici PAC 1.0 cultivated in MRS broth Ion exchange chromatography, hydrophobic interaction chromatography 73 – Beaulieu et al. [14]
2 Pediocin PD-1 Pediococcuc damnosus NCFB 1832 isolated from beer Ammonium sulphate precipitation, cation exchange chromatography 34 1700 Bauer et al. [13]
2 Pediocin NCDC35 Pediococcus pentosaceus NCDC35 Ammonium sulphate precipitation, ion-exchange chromatography 14.4 14.01 Meena et al. [92]
2 Paracasin SD1 Lactobacillus paracasei SD1 Ammonium sulfate precipitation, gel filtration chromatography – 871 P Wannun et al. [150]
3 Enterocin TJUQ1 Enterococcus faecium TJUQ1 isolated from pickled Chinese celery Ammonium sulfate precipitation, reversed-phase chromatography (Sep-Pak C 8) and cation-exchange chromatography – 35.89 Qiao et al. [118]
3 Enterocin LD3 Enterococcus hirae LD3 Ammonium sulphate precipitation, ion-exchange chromatography, gel filtration chromatography 0.37 13.2 Gupta et al. [57]
3 Plantaricin GZ1-27 Lactobacillus plantarum GZ1-27 Ammonium sulphate precipitation, gel-filtration chromatography, and RP-HPLC 0.1 10.55 Pei et al. [110]
3 Bacteriocin LF-BZ532 Lactobacillus fermentum BZ532 Ammonium sulphate precipitation, cation-exchange chromatography, and RP-HPLC 0.85 4.2 Rasheed et al. [121]
3 Fermencin SD11 Lactobacillus fermentum SD11 Ammonium sulfate precipitation, gel filtration chromatography, and reverse-phase high-performance liquid chromatography – 7,526 Phirawat Wannun et al. [151]
3 Plantaricin KL-1X Lactobacillus plantarum KL-1 Ammonium sulphate precipitation, ion-exchange chromatography, RP-HPLC 0.004 0.04 Rumjuankiat et al. [127]
  KL-1Y    0.8 10.5  
  KL-1Z    0.1 0.9  
3 Sakacin A Lactobacillus sakei 2675 Ion exchange chromatography, hydrophobic interaction chromatography, RP-HPLC 10 – Guyonnet et al. [58]
  Sakacin P Lactobacillus sakei 2525   50 –  
  Enterocin A Enterococcus faecalis 336   66 –  
  Pediocin PA-1 Pediococcus acidilactici 1521   25 –  
4 Bacteriocin-zrx01 Lactobacillus rhamnosus zrx01 isolated from Kefir Ammonium sulphate precipitation, ion-exchange chromatography, gel filtration chromatography, RP-FPLC 1.76 8.5 Pei et al. [110]
4 Paracin 54 Lactobacillus paracasei ZFM54 Ammonium sulphate, strong cation-exchange chromatography, G-25 gel column, and RP-HPLC 1.93 45.64 Zhu et al. [161]
4 Enterocin HDX-2 Enterococcus faecium HDX-2 isolated from pickled Chinese cucumber Ammonium sulphate precipitation, ion-exchange chromatography, hydrophobic interaction chromatography, RP-HPLC 6.38 56.61 Du et al. [43]
4 Plantaricin ZJ5 Lactobacillus plantarum ZJ5 Ammonium sulphate precipitation, ion-exchange chromatography, hydrophobic interaction chromatography, RP-HPLC 1.7 139.5 Song et al. [138]
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