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The evaluation of healing effect of low-level laser treatment following gingivectomy



Lasers have been investigated as an alternative or adjunctive tool to conventional procedures commonly used in periodontology. The application of low-level laser therapy (LLLT) in dentistry was proposed to provide minimally invasive and painless treatment as well as increasing the comfort for the patient following surgery. Thus, the purpose of the current study was to evaluate the effect of LLLT on healing following gingivectomy.


Twenty patients with inflammatory gingival enlargement on their maxillary or mandibular anterior region were included in this study. Patients were divided randomly into two groups (test group consist of ten patients) after gingivectomy a diode laser (660 nm) was applied to the wound area immediately post-surgery, 3 and 5 days post-surgery. The control group (ten patients) did not receive laser irradiation. Healing was evaluated clinically using healing score and visual analogue scale (VAS).


The laser-treated group had significant improvement regarding the healing score at 5, 7, and 14 days p.s., and significant improvement in visual analogue scale (VAS) score was observed at day 3, 5, and 7 p.s., than the control group.


LLLT can be used as an effective adjunctive treatment following gingivectomy procedure to promote healing and decrease patients’ discomfort.


More than 40 years, lasers were considered as adjunctive therapy to conventional procedures frequently used in periodontology. Many lasers such as CO2, Er:YAG, and diode have been used for resective surgeries due to their rapid and hemostasis effect [10, 22, 38, 44]. Also, they have been used for non-surgical applications as sulcular debridement of pockets [3] and the laser-assisted new attachment procedure (LANAP) [37].

Although solid lasers, such as neodymium yttrium aluminum garnet (Nd: YAG) and Er:YAG, can be utilized for hard and soft tissue applications, the unpredictable penetration depth and energy density inside the tissues have raised concerns. Alternatively, low-level laser therapy (LLLT) is indicated for a wide variety of procedures in dental practice based on the comfort, efficiency, and decreased cost than hard lasers [5, 35, 40, 43].

The extensive use of LLLT in dentistry depends on the appraisals of patient preferring superficially invasive and painless technique as well as increasing his comfort following surgery [25, 31]. The action of low-level lasers is due to its biostimulatory effect it exert on various cells [6, 34]. Previous studies have reported that LLLT exert biologic effects on tissues in many ways such as stimulating fibroblast maturation and motility that subsequently, enhance cell metabolism and proliferation [16, 23]

Previous studies observed that LLLT promotes fibroblast and keratinocyte cell locomotion [18, 42, 45, 46], collagen formation [26], growth factor production and angiogenesis [39]that result in enhancement of wound healing [4].

LLLT using laser devices such as helium–neon, diode, and gallium arsenide have been applied for different oral conditions. Treating mucositis [21], paresthaesia [17], and TMJ disorders [42] have been investigated. Furthermore, LLLT has been used for reducing post-operative discomfort after gingivectomy [6, 30], endodontic surgery [19], enhance healing, and dentinogenesis following pulpotomy [41] and as an adjunct to non-surgical periodontal therapy [20, 27, 28].

The aim of the current study was to evaluate the effectiveness of LLLT on gingival healing following gingivectomy procedure.

Subjects and methods

Study design and patient selection

The study sample was selected from patients referred to the Department of Periodontology, Alexandria University, between September and December 2015. Inclusion criteria were systemic healthy patients aged from 22 to 45 years and diagnosed with chronic inflammatory gingival enlargement, non-pregnant and non-smoker. Gingivectomy procedure for maxillary or mandibular anterior region with at least six affected teeth was indicated.

Pre-surgical preparation for all subjects consisted of supra- and sub-gingival scaling, oral hygiene instructions and occlusal adjustments if needed. Initial probing depths were recorded for six sites for each affected tooth using William’s probe (Hu-Friedy). After 4 weeks at re-evaluation phase, the gingival condition of the patients was re-evaluated prior to the gingivectomy procedure.

Ethical approvals

The study protocol was approved by the internal review board of the Faculty of Dentistry, in accordance with the Declaration of Helsinki, and signed written consents were taken from the patients prior to the study.

Surgical procedure

Randomly, the patients were divided into two groups; the test group (10 patients, with mean age 37 ±2 years) in which LLLT was applied after the gingivectomy. While in the control group (10 patients, with mean age 35 ± 3 years), no LLLT was used following the gingivectomy [16, 30] (Figs. 1 and 2).

Fig. 1

Clinical view of gingival enlargement in mandibular incisors area indicated for gingivectomy (test group). a Before gingivectomy procedure. b Immediately after gingivectomy (white arrows). c Application of low-level laser therapy (LLLT) to the surgical site. d Gingival healing 2 weeks post-surgery. Note the change in gingival contour after surgery (white arrows in b) and 2 weeks later (swhite arrows in d)

Fig. 2

Clinical view of gingival enlargement in mandibular incisors indicated for gingivectomy (control group). a Before gingivectomy procedure. b Immediately after gingivectomy. c Gingival healing 2 weeks post-surgery. Note the change in gingival contour after surgery (white arrows in b) and 2 weeks later (white arrows in c)

The gingival pockets were first examined with a periodontal probe and marked with a pocket marker, then Kirkland knife was used for external bevel incision and Orban’s knife was used for releasing the interdental tissue.

Laser protocol

After hemostasis, the surgical site of the test group was subjected to laser irradiation using diode laser (photon laser comp. 660 nm) 50–mW output power, continuous wave, and beam diameter of 3 mm immediately after surgery and at days 3 and 5 p.s. The delivery tip was perpendicular to the gingival tissue surface at 1 cm distance in a scanning mode of an area about 1 cm2 for 80 s (4 J/cm2 energy density). Periodontal dressing (Coe-Pak) was applied on the surgical sites for both groups. A titanium foil was placed in the inner surface of the pack to prevent mechanical injury to the tissues during healing and during pack removal for further laser applications every 48 h for 1 week. In order to minimize inter-operator variations, the same periodontist (M.M) performed all surgical procedures.

Clinical assessment

Photographs of the surgical sites at pre-surgery, immediately post-surgery, and at 3, 5, 7, and 14 days p.s. were taken. Two examiners assess the photographs to score three clinical parameters. These examiners were blinded to test and control pictures.

Clinical parameters consisted of tissue color (pink, red, bluish), tissue contour (normal, hyperplastic, atrophic), and clinical status of the healing wound for the specific time interval (normal, better than normal, worse than normal). A three-stage scale was used to assess the wound healing:

A) Healing score [19]

Score 0: control and laser-treated sites showed the same healing extent.

Score 1: better healing was observed for control than laser treated sites.

Score 2: better healing was observed for laser treated than control sites.

B-Visual analogue scale scores (VAS) [14]

The visual analogue scale is a continuous scale consists of a horizontal line of 10 cm in length with two end-points. Score of 0 represents “no pain” while score 10 is for “worst pain.” Patients recorded the severity of pain on the scale (0 no pain; 1–3 slight pain; 4–6 moderate pain; 7–10 severe pain) at four time points (immediately after treatment, 3, 5, and 7 days post-operatively).

Statistical analysis

Normality of data was evaluated using Shapiro–Wilk test. The nonparametric Mann-Whitney U test was used to compare between laser and control groups regarding the healing score, while t test was used for VAS score comparison. Significance was accepted at p < 0.05. The nonparametric kappa test was used to evaluate agreement between the two examiners regarding the healing score.


Clinical results

Healing score

Both groups showed similar results immediately post-surgery. At day 3 p.s., a better improvement was observed in wound healing for laser than control group. Significant improved healing for laser than control group was detected at 5, 7, and 14 days p.s. Significant difference was observed between day 1 and 5 (z = − 2.6, p = 0.009), 7 (z = − 2.98, p = 0.002), and 14 (z = − 2.9, p = 0.002) (Fig. 3). The kappa index of scoring agreement between the examiners was 0.671.

Fig. 3

Graphical presentation of gingival wound healing for laser (test) and control groups during the study period. At day 1, all cases showed same healing (score 0). Starting from day 3, better healing was observed in laser than control group (score 2). Score 0: control and laser-treated sites showed the same healing extent. Score 1: better healing was observed for control than laser treated sites. Score 2: better healing was observed for laser treated than control sites. (* significant difference, p < 0.05)


Both groups showed similar results at day 1 (t1 = − 0.80178, p value = 0.211). At day 3, 5, and 7 p.s., a significant reduction in VAS score was shown for laser compared to the control group (t3 = − 4.9, p = 0.0004, t5 = − 6.1 p = 0.0001, t7 = − 2.05, p = 0.027) (Table 1).

Table 1 Visual analogue scale scores (VAS) for laser and control groups during the study period


The perceptions of patients’ desire regarding painless minimally invasive treatment have gained LLLT a lot of interest in field of dentistry [25]. Previous studies described the application of LLLT for management of oral and periodontal conditions [1,2,3,4].

Previous studies showed that low-level laser therapy (LLLT) regulates many cellular functions, increase ATP synthesis, and enhances the healing activity [15, 34].

Different LLLT parameters have been used to assess its influence on wound healing. Laser has shown effectiveness in modulating cellular events in vitro that leads to accelerated healing process. However, no consensus on specific laser parameter was reached [32].

Our findings showed that LLLT positively enhance wound healing following the gingivectomy procedure as well as decrease the post-operative pain perception by patients. This is in agreement with Oncu et al. [24]; they showed that diode laser significantly accelerate healing and decrease patients’ discomfort following gingivectomy.

In this study, the selected parameters were 4 J/cm2 and exposure time of 80 s that were applied immediately following surgery and repeated on alternative days for 1 week. These parameters have been previously investigated and showed favorable results [32].

Our findings are in contrast to Damante et al. [6] in which they showed that LLLT did not improve healing following gingivoplasty. This could be due to the different laser settings that were used than in our study. Damante et al. [6] used single application of 15 mW GaAlAs laser in a punctual mode on the right side of 16 patients while in our study a 50-mW GaAlAs laser was applied in scanning mode over the wound area every 48 h for 1 week.

Previous studies [36, 39] showed that production of fibroblast growth factors by macrophages and fibroblasts was boosted following LLLT application. It has been suggested that LLLT application improved wound healing through increasing keratinocytes’ proliferation, stimulating early epithelization and improving neovascularization. Following gingivectomy, collagen production and organized tissue alignment occur gradually within 3–4 weeks [29]. Thus, acceleration of the wound healing process with laser can be interpreted by a greater collagen production and vascular proliferation in the connective tissue combined with higher proliferative activity in epithelial cells

LLLT has been found to promote growth factors release in the blood stream, which can reach adjacent and distant sites of the body [6]. Therefore, it can be speculated that the application of laser to one site in the mouth may affect the adjacent site as well. In order to avoid this systemic effect of laser therapy we used case-control individuals in the current study and not split mouth design .

The mechanisms by which LLLT acts are complex but mainly depends on the stimulation of normal cell functions that is induced by low levels of laser energy. This result is due to modifying the mitochondrial respiratory chain or the calcium channels [13, 33] which consequently stimulates cell proliferation and metabolism [16]. Enhancement of cell proliferation and decreasing the levels of inflammatory cytokines, the healing of the oral mucosa would be accelerated [2]. Previous studies demonstrate the ability of LLLT in stimulating human fibroblasts, immune cells, and epithelial cells along with enhanced angiogenesis, growth factor release, and decreasing post-operative pain which ultimately leads to improved wound healing [12, 27].

The laser group showed significant better healing score than the control group at 5, 7, and 14 days p.s. This could be due to the increase in revascularization that accelerate healing following LLLT [8].

In the current study, the highest VAS reduction percentage was observed in laser group. This comes in agreement with Reddy et al. [30] who noted a significant decrease in pain score for the laser group especially in the first 2 days following surgery compared to conventional treatment. Also, Enwemeka et al. [9] in their meta-analysis concluded that laser phototherapy may be considered a highly efficient therapeutic approach for pain relief and tissue repair. Silveira et al. [34] suggested that low-level laser application increase ATP production that hasten the healing process of muscles.

Our finding agrees with previous reports [1, 7, 11] in which they noticed that 75% of patients suffering from recurrent apthous ulcer RAS recorded reduced pain perception immediately following laser application than those who used topical steroids.

Similarly, Hersheal et al. [11] found faster healing and reduced pain after using laser application on RAS. They concluded that LLLT represent the most beneficial treatment for minor RAS. However, longitudinal clinical trials are further recommended to explore the advantages of LLLT in treatment of various oral conditions.


Low-level laser as an adjunctive therapy to gingivectomy procedure can be used to decrease patients’ pain perception following surgery and accelerate the healing process especially in the early phases.

Availability of data and materials

Data is available upon request from authors.



Low-level laser therapy


Visual analogue scale


Gallium aluminum arsenide


Recurrent apthous ulcer


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M M: carried out the clinical part. MM and M M M carried out the manuscript writing and results analysis. All authors have read and approved the manuscript.

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Correspondence to Marwa Madi.

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The protocol of the study was approved by the internal review board (IRB no.: 00010555) of the Faculty of Dentistry, Alexandria University, in accordance with the Declaration of Helsinki, and signed written consents were taken from the patients prior to the study.

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Madi, M., Mahmoud, M.M. The evaluation of healing effect of low-level laser treatment following gingivectomy. Beni-Suef Univ J Basic Appl Sci 9, 25 (2020).

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  • Gingivectomy
  • Low-level laser
  • Healing