A review on the efficacy of Ocimum gratissimum, Mentha spicata, and Moringa oleifera leaf extracts in repelling mosquito
Beni-Suef University Journal of Basic and Applied Sciences volume 10, Article number: 87 (2021)
@@@In recent times, repellents and synthetic drugs have been identified as having negative toxicity effects on humans and the environment. Apart from the unfavourable effects on man and livestock caused by these chemicals-based (synthetic) repellents, they are also expensive, non-biodegradable, and no more effective because mosquitoes are getting adapted. With these drawbacks, an eco-friendly plant-based insecticide as a substitute is needed urgently. This paper reviews the extraction and use of essential oil from the leaves of Mentha spicata, Ocimum gratissimum, and Moringa oleifera as mosquito repellent. Carvone, Eugenol, and 9-Octadecenoic acid were discovered to be the most active components in the M. spicata, O. gratissimum, and M. oleifera extracts, respectively, using gas chromatography-mass spectrometry (GC-MS).
In recent times, repellents and synthetic drugs have been identified as having negative toxicity effects on humans and the environment. Apart from the unfavorable effects on man and livestock caused by these chemical-based (synthetic) repellents, they are also expensive, non-biodegradable, and no more effective because mosquitoes are getting adapted.
An eco-friendly plant-based insecticide as a substitute is needed urgently.
Diseases transmitted by mosquitoes are still a significant reason for the global mortality rate, with over 700 million individuals experiencing such diseases every year.
With the proper formulation of other repellent forms using their oils, they can replace non-degradable synthetic mosquito repellents since they are eco-friendly. In general, the mosquitocidal activity and percentage protection of plant extract increase with increasing concentration of the extracts in different formulations.
This paper is our original work. We certify that this manuscript has not been published in part or whole elsewhere in any language, and it has not been submitted to any other journal for reviews.
Mosquitoes cause inconvenience by their bites, as well as the transmission of deadly diseases . Fewer than 10% of the roughly identified mosquito species are viewed as proficient vectors and pathogenic carriers of infections with significant effects on human health and well-being, both directly and indirectly.
Diseases transmitted by mosquitoes are still a significant reason for the global mortality rate, with over 700 million individuals experiencing such diseases every year .
Diseases carried by mosquitoes affect the economy, incorporating loss in business and work yields, especially in nations with marine and temperate atmospheres; however, anywhere in the world is prone to vector-borne infections . Various diseases like; Filariasis, Malaria, yellow fever, Japanese encephalitis, zika virus, Dengue fever, etc., are transmitted by mosquitoes . Malaria is among the biggest public health issues globally. Especially in parts of Africa, in which Nigeria has the largest amount of cases of malaria .
However, over time, the constant application of synthetic insecticides has culminated in mosquito resistance. This is due to their adaptation and ability to develop resistance to the active ingredient of the chemicals and also changes in its metabolism and behaviour. Mosquitoes have compound forms of identifying their hosts, and various types of mosquitoes react to various stimuli. Some mosquitoes are active in the dawn and dusk, but during the day, there are also mosquitoes searching for hosts . In other to prevent being bitten by mosquitoes, materials, or actions that attract them are advised to be avoided even as their repellents are being applied. And also, the acts that reduce the repellent's effectiveness should be averted .
Mosquito repellents generally function by hindering the capacity of the female mosquito to recognize the external stimuli (for example, Carbon-dioxide, water vapor, and heat) that she utilizes to spot a host . Various synthetic and plant-based chemicals are available and are known to ward off mosquitoes. N,N-Diethyl-meta-toluamide, also called DEET, is the most frequently used synthetic chemical repellent. Since it became commercially available, it has been implemented above one million times in the 40+ years of its existence. DEET-based insect repellents cause irreparable harm to the environment since they consist of chemicals that are not easily degraded, and their associated neurotoxicity . An alternative to repelling mosquitoes could be plant-based natural materials like plant oils to prevent the adverse effects of synthetic repellents. In comparison with synthetic repellents, they are deemed safe and good for the environment .
Before the application of synthetic substances, the repellent features of plants on mosquitoes as well as other pest insects were well known. Humans historically used natural compounds to shield themselves from insect bites . Since ancient times, man has utilized plant parts and secondary plant metabolites for pest control. During the pre-DEET period, vector mosquito reduction relied primarily on the environmental control of the breeding surroundings, i.e., depleting the source. During this time, Pyrethrum, Anabasine, Quassia, Camphor, Turpentine, Nicotine, Derris, D-limonene, Hellebore, Azadirachtin, and Chrysanthemum were some plant-based (botanical) insecticides used in different countries .
As a possible source of insecticidal materials, the plant kingdom has been of considerable interest. Many plant kingdom species produce a variety of secondary metabolites that perform a crucial role in plant defence against mosquitoes/insects. Plants are a great source of biologically active chemicals/compounds and can be a substitute source for mosquito repellent products . Furthermore, unlike regular insecticides that consist of a sole active ingredient, insecticides derived from plants contain botanical mixtures of chemicals/compounds that work collectively on processes, both physiological and behavioural .
Biologically active compounds derived from selected plants species such as Ocimum gratissimum (O. gratissimum), Hyptis sauveolen (H. sauveolen), Acarcia arabica, Azadirachta indica, and Eleusive indica have been commonly used in the past to control insects in many tropical counties . The use of compounds from plants for controlling mosquitoes has been accounted for since 1933, and these chemicals in their essential oils obtained from the various plants have shown to be a feasible alternative to synthetic repellents and also exhibit good repellence against mosquitoes . Extracts and essential oil from plants can be a replacement for synthetic insecticides since they are efficient, easily decomposable, environmentally friendly, and relatively cheap.
Essential oils, which are volatile substances, have an oily scent and are derived from the various parts of the plant . They are extracted with diverse methods, and plant parts used for their isolation include leaves, stem bark, flowers, and roots .
2 Main text
2.1 Forms of mosquito repellent
Aerosol—The most commonly used form of repellents for mosquito is aerosol. It comprises of a propellant, a solvent, and other components. The active ingredient is diluted to a particular concentration by ethanol or propyl alcohol, the solvent. It also holds the mixture of all the materials, so that even after extended storage, the product will still be active. They are packed and discharged as a spray under pressure .
Cream—Repellent creams for mosquito make the individual unattractive to the biting mosquitoes. The smell of the skin and human breathe attracts mosquitoes and other insects that feast on blood. Carbon dioxide is released by humans once they breathe out, and this draws insects. The skin is rendered unattractive to mosquitoes by the application of this repellent cream Thus, the mosquitoes will be seen flying around, but it will not bite you .
Stick—These repellent incense sticks are plant-based and DEET-free. The sticks use a combination of citronella, rosemary, peppermint, lemon grass, cedar wood, and bamboo to ward off mosquitos . They release fragrant smoke when they are burnt, which also wards off mosquitoes around the place of burning.
Mosquito coil—A mosquito coil is an incense for repelling mosquito, usually shaped into a spiral. It is generally kept in the middle of the spiral, holding it in the air. Its working mechanism is also identical with the incense stick, and candle as its burning normally starts at the far end of the spiral and moves toward the middle of the spiral, creating smoke that repels mosquitoes .
There are many mosquito repelling plants, both wild and cultivated. It is imperative to note that the repelling is carried out by compounds situated inside the plants. Common plants that repel mosquitoes include; Citronella plant, lemon grass, lavender, mint, catnip, rosemary, garlic, basil, floss flower, beebalm, etc.
2.2 Mentha spicata
Mentha spicata, also known as spearmint, is a genus from the Lamiaceae (mint) family, and plants belonging to this family are a great source of polyphenols with good antioxidant features . Spearmint essential oils have often been utilized in different ways, like plant diseases and insect pest control, in conventional medicine, and also in cosmetics and culinary . Spearmint is native to northern England and is cultured in tropical to moderate climate zones like Europe, South Africa, America, China, and Brazil. These days, it is grown extensively all around the world . Because of its invasive, creeping rhizomes, gardeners usually plant it in containers or planters, and its leaves can still be used clean, frozen, or dry .
The most copious chemical in spearmint oil is R-carvone, giving its distinctive scent to spearmint [25, 26]. Spearmint essential oil was successful as a mosquito larvicide. Because of its toxicity and negative environmental effect, the application of spearmint as a larvicide would be a better substitute for conventional insecticides . Carvone is noted to have the potential of preventing the growth of bacteria, and also acting as an insect repellent and fungicide . Spearmint leaves were chosen because its essential oil is a good natural insect repellent from past work, and its distinctive smell will improve the quality of the repellent forms (Fig. 1).
2.3 Moringa oleifera
One of the most commonly grown species of a mono-generic family—the Moringaceae is Moringa oleifera (M. oleifera), and it’s indigenous to India’s sub-Himalayan regions [27, 28]. Moringa is cultivated predominantly in semi-arid, tropical, and temperate regions. In dry sandy soil, it thrives best, tolerating poor soil, and also in coastal regions. It develops in nearly all types of well-drained soils and is resistant to drought and retains water throughout the dry season by shedding of leaves. It is usually grown and naturalized in Mexico, Central and South America, Sri Lanka, tropical Africa, Philippines, India, and Malaysia. .
The Moringa tree is deemed among the world’s most valuable trees; nearly every portion can be used for nutrition or contains some beneficial property. It is a conventional food crop that has the potentials of improving nutrition, boosting food protection, fostering rural growth, and promote sustainable land management . Every part of Moringa has been effectively used against various ailments. Extracts from its leaf exhibit antioxidant and hypo-cholesterolaemic activities [31, 32].
The phytochemicals present in M. Oleifera can function as larvicides, repellents, arthropod growth controllers, and also possess a very deterrent behaviour as noticed by many researchers and analysts. Seed extract from M. Oleifera has a good effect on malaria and has no adverse impact on humans . Moringa plant extracts possess repellent properties and have an extensive range of medicinal applications. Different portions of this plant, like the leaves, fruit, seed, flowers, bark, and roots, contain a list of important purposes  (Fig. 2).
2.4 Ocimum gratissimum
Ocimum gratissimum, also called basil clove, African basil, is an Ocimum species from Africa, South Asia, and South America . It is a species of tropical plants popularly referred to as “scent leaf,” as Nigerians love to call it. In native Nigeria, it is called Nchanwu leaf in (Igbo), Daidoya in (Hausa), and Efirin in (Yoruba). It is a tropical plant species belonging to the Labiatae family . The leaf consists of a variety of nutrients and minerals that provide the plant with many health benefits . It is stated that fresh basil leaves contain protein, magnesium, Anatol, Boron compounds, Eugenol, Tryptophan, Stigmasterol, zinc, Tannin, and Cinnamic acid .
They are often used to make soups as a local spice and flavor. O. gratissimum's essential oil primarily contains eugenol, which provides some signs of antibacterial activity as well as a strong mosquito repellent due to its smell and insect toxicity [39, 40]. Thus, Scent leaf can be mixed into mosquito coils, incense, creams, or ointments to ward off reptiles and insects .
It is an herbaceous plant that grows with an upright stem reaching six feet high . Scent leaf, which is a potential repellent plant, was considered for the study as it is cheap, easily accessible (as can be found in the backyard of most homes or local markets) (Fig. 3).
2.5 Essential oil and extraction
The essential oil is a potent water repellent liquid that houses labile plant-based chemical compounds (easily evaporated at normal temperatures) [44, 45]. Often known as volatile oils, they are also named according to the plant they were derived from, like clove oil, which is an essential oil obtained from the clove . The metabolites such as the monoterpenes like camphor, eugenol, citronellol, terpinolene, α-pinene, citronellal, thymol, limonene, and cineole are the usual components in several essential oils showing mosquito repellent behaviour .
Extraction "means transferring compounds from a liquid or solid to another solvent or phase . Two immiscible phases are combined to separate a solvent from one phase to the next, depending on the relative solubility in each phase. Extraction is a primary method used in plant materials for separating their compounds. Since essential oils are the liquefied form of the plant, instead of being produced in laboratories synthetically, they are obtained from materials of a plant by extraction methods appropriate for the specific plant component containing the oils. The common extraction methods for essential oil include; Soxhlet extraction, steam distillation, CO2 extraction, water distillation, maceration, cold press extraction, and effleurage [48, 49].
2.6 Characterization of the essential oils
Characterization involves the description of the distinctive nature or features of the extracted oils. Characterization is based on different analyses for the extract, such as phytochemicals, gas chromatography-mass spectrometry [GC-MS], and Fourier Transform Infrared Spectroscopy [FTIR] analysis.
2.7 GC-MS analysis
One of the presumed hyphenated analytical techniques is gas chromatography-mass spectroscopy (GC-MS). GC-MS is an analytical tool incorporating the features of gas chromatography and mass spectrometry to determine various components in a test sample . Gas chromatography isolates the components of a mixture, and each component is individually identified by mass spectroscopy.
GC-MS is used to analyse organic compound mixtures that are unknown, and its application in determining the composition of bio-oils extracted from raw biomass is a key use of this technology .
2.8 FTIR analysis
Fourier-transform infrared spectroscopy (FTIR) is used to acquire from a solid, liquid, or gas sample its infrared absorption or emission spectrum. At the same time, an FTIR spectrometer obtains data with high spectral resolution over a broad spectral range . According to , “FTIR screening is essentially an experimental analysis technique used to distinguish organic and some inorganic substances by applying infrared radiation (IR).” The FTIR instrument delivers about 10,000 to 100 cm−1 infrared radiation over a sample, absorbing some radiation and passing through some. Vibrational energy is produced by the conversion of absorbed radiation .
Table 1 shows the comparative analysis of the three (3) plant leaf extracts (O. gratissimum, M. spicata and M. oleifera) with other leaf extracts as a repellent for mosquito while the functional groups showing various functional groups found in the oils were reported in Tables 2, 3, and 4, respectively. The FTIR spectrum confirmed the presence of alcohols, phenols, alkanes, alkenes, carbonyl, carboxylic acids, and aromatic compounds in M. spicata extract. Alcohols, amines, alkanes, ketones, carbonyl, nitro, and aromatics were the major functional groups present in the M. oleifera leaf extract. While the FTIR spectrum of O. gratissimum leaf extract revealed the presence of the following functional groups; Amines, Alkanes (-CH3), Phosphorus/Organo sulphur compounds, Alpha–Halogenonitro Compound, Alcohols, Nitrates, Benzene ring, and Haloids.
From the GC-MS analysis (chemical composition) of the essential oils of the three leaves, 44 compounds were found in O. gratissimum, 16 compounds in M. oleifera, and 33 compounds in M. spicata, as shown in Tables 5, 6 and 7, respectively. The active compound/components of the three extracts are Eugenol (61.9%), 9-Octadecenoic acid (20.89%), and Carvone (56.4%) for O. gratissimum, M. oleifera, and M. spicata, respectively. The result from Table 7 which shows Carvone having the highest composition and most prevalent in M. spicata oil corroborates with the result of  in which carvone was discovered as the most sufficient compound/component in the extract. The extracts also showed the presence of various biologically active phytocomponents in the GC-MS analysis. The presence of these photo components also contributes to the observed medicinal property in addition to the antimicrobial activity of the plant. Among the three leaves, O. gratissimum extract is the best repellent plant, followed by M. spicata and M. oleifera. This is due to the high composition of eugenol (which is a phenol) in the extract as it has been reported not only to control insects like mosquitoes but also to provide a knock-out effect on them . The active component of clove oil, eugenol, is a fast-acting contact insecticide that is effective on a wide variety of insects/pests such as mosquitoes, ants, cockroaches, etc. . Eugenol has little or no residual activity other than a lingering scent of cloves as mosquitoes detest the smell of cloves . 9-Octadecenoic acid, which is the most prevalent compound in Moringa leaf oil extract, exhibited moderate repellent activity at 30 min after treatment, according to . According to , leaf extracts of M. oleifera leaves possessed poor repellent activity, and this corroborates with the result of .
According to , M. oleifera leaves offered 58% protection from its smoke when incorporated into a coil while 70.37% protection was offered by O. gratissimum coil according to  at moderate concentration. Therefore, the best repellent plant and repellent form from the sample leaves for repelling mosquitoes is O. gratissimum leaf in a coil.
The initial findings of the laboratory assessment from the previous works show the repellent potential of M. spicata, O. gratissimum, and M. oleifera leaves against mosquitoes. With the proper formulation of other repellent forms using their oils, they can replace non-degradable synthetic mosquito repellents since they are eco-friendly. In general, the mosquitocidal activity and percentage protection of plant extract increase with the increasing concentration of the extracts in different formulations.
The Soxhlet extraction technique is a conventional and most preferable method for obtaining plant extracts easily. However, the result from the efficacy tests revealed that natural repellents derived from plant extracts tend to provide protection for a shorter time. The active component of the M. spicata extract responsible for its repellent activity is carvone. While that of O. gratissimum and M. oleifera are Eugenol and 9-Octadecenoic acid, respectively. O. gratissimum essential oil is the best repellent plant, and its incorporation into a mosquito coil will offer the best protection against mosquitoes in comparison with the other plants and repellent forms.
Finally, the study establishes and reaffirms the potential of applying indigenous Nigerian plants’ oil extracts with insecticidal properties for Mosquito control.
Availability of data and materials
Data sharing is not applicable to this article as no datasets were generated or analyzed during the current study.
Gas chromatography-mass spectrometry
Fourier-transform infrared spectroscopy
Foster WA, Walker ED (2019) Chapter 15—mosquitoes (Culicidae). Medical and veterinary entomology, 3rd edn. Academic Press, Cambridge, pp 261–325. https://doi.org/10.1016/B978-0-12-814043-7.00015-7
Madhubabu G, Yenugu S (2017) Exposure to allethrin-based mosquito coil smoke during gestation and postnatal development affects reproductive function in male offspring of rat. Inhal Toxicol 29:374–385. https://doi.org/10.1080/08958378.2017.1385661
Fradin MS (2002) Day JF (2002) Comparative efficacy of insect repellents against mosquito bites. N Engl J Med 347(1):13–18. https://doi.org/10.1056/NEJMoa011699
Ojewumi ME, Adeyemi AO, Ojewumi EO (2018) Oil extract from local leaves—an alternative to synthetic mosquito repellants. Pharmacophore 9:1–6
Dawaki S, Al-Mekhlafi HM, Ithoi I, Ibrahim J, Atroosh WM, Abdulsalam AM et al (2016) Is Nigeria winning the battle against malaria? Prevalence, risk factors and KAP assessment among Hausa communities in Kano State. Malar J 15:1–14. https://doi.org/10.1186/s12936-016-1394-3
Blythe EK, Tabanca N, Demirci B, Tsikolia M, Bloomquist JR et al (2016) Lantana montevidensis essential oil: chemical composition and mosquito repellent activity against Aedes aegypti. Nat Prod Commun 11:1934578X1601101122
Peairs FB, Cranshaw WS (1991) Mosquito management. Doctoral dissertation, Colorado State University. Libraries
Fradin MS (1998) Mosquitoes and mosquito repellents: a clinician’s. Ann Int Med 128:931–940
Azeem M, Zaman T, Tahir M, Haris A, Iqbal Z et al (2019) Chemical composition and repellent activity of native plants essential oils against dengue mosquito, Aedes aegypti. Ind Crops Prod 140:111609. https://doi.org/10.1016/j.indcrop.2019.111609
Prophiro JS, da Silva MAN, Kanis LA, da Silva BM, Duque-Luna JE, da Silva OS (2012) Evaluation of time toxicity, residual effect, and growth-inhibiting property of Carapa guianensis and Copaifera sp. in Aedes aegypti. Parasitol Res 110:713–719. https://doi.org/10.1007/s00436-011-2547-5
Shaalan EAS, Canyonb D, Younesc MWF, Abdel-Wahaba H, Mansoura AH (2005) A review of botanical phytochemicals with mosquitocidal potential. Environ Int 31:1149–1166. https://doi.org/10.1016/j.envint.2005.03.003
Mgbemena IC, Ebe T, Nnadozie AI, Ekeanyanwu KK (2015) Repellent activities of the methanolic leaf extracts of Moringa oleifera and Stachytarpheta indica against Aedes aegypti mosquito. J Pharm Biol Sci 10:77–81. https://doi.org/10.9790/3008-10427781
Ghosh A, Chowdury N, Chandra G (2012) Plant extracts as potential mosquito Larvicides. Indian J Med Res 135:581–598
Ojewumi ME, Owolabi RU (2012) The effectiveness of the extract of ‘hyptis sauveolens’ leave (a specie of effinrin) in repelling mosquito. Trans J Sci Technol 2:78–87
Govindarajan M, Mathivanan T, Elumalai K, Krishnappa K, Anandan A (2011) Ovicidal and repellent activities of botanical extracts against Culex quinquefasciatus Aedes aegypti and Anopheles stephensi (Diptera: Culicidae). Asian Pac J Trop Biomed 1:43–48. https://doi.org/10.1016/S2221-1691(11)60066-X
Ojewumi ME, Adedokun SO, Omodara OJ, Oyeniyi EA, Taiwo OS, Ojewumi EO (2017) Phytochemical and antimicrobial activities of the leaf oil extract of Mentha spicata and its efficacy in repelling mosquito. Inter J Pharm Res Allied Sci 6:17–27
Irshad M, Subhani MA, Ali S, Hussain A (2019) Biological importance of essential oils. Essent Oils Oils Nat. https://doi.org/10.5772/intechopen.87198
Gallegos C, Franco JM (1999) Rheology of food, cosmetics and pharmaceuticals. Curr Opin Colloid Interface Sci 4:288–293. https://doi.org/10.1016/S1359-0294(99)00003-5
Enserink M (2008) A mosquito goes global. Science 320:864–866. https://doi.org/10.1126/science.320.5878.864
Solomon B, Sahle FF, Gebre-Mariam T, Asres K, Neubert RH (2012) Microencapsulation of citronella oil for mosquito-repellent application: formulation and in vitro permeation studies. Eur J Pharm Biopharm 80:61–66. https://doi.org/10.1016/j.ejpb.2011.08.003
Liu W, Zhang J, Hashim JH, Jalaludin J, Hashim Z, Goldstein BD (2003) Mosquito coil emissions and health implications. Environ Health Perspect 111:1454–1460
Kee LA, Shori AB, Baba AS (2017) Bioactivity and health effects of Mentha spicata. Integr Food Nutr Metab 5:1–2. https://doi.org/10.15761/IFNM.1000203
Singh P, Pandey AK (2018) Prospective of essential oils of the genus Mentha as bio pesticides: a review. Front Plant Sci 9:1295. https://doi.org/10.3389/fpls.2018.01295
Lyen K (2020) Public enemy number one: the mosquito. INSIGHT. SMA NEWS, pp 25–27
Yogalakshmi K, Rajeswari M, Sivakumar R, Govindarajan M (2012) Chemical composition and larvicidal activity of essential oil from Mentha spicata (Linn.) against three mosquito species. Parasitol Res 110:2023–2032
Ojewumi ME, Adedokun SO, Ayoola AA, Taiwo OS (2018) Evaluation of the oil extract from Mentha spicata and its chemical constituents. PONTE Int J Sci Res 74:68–89. https://doi.org/10.21506/j.ponte.2018.11.7
Moyo B, Masika PJ, Hugo A, Muchenje V (2011) Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. Afr J Biotechnol 10:12925–12933
Ojewumi ME, Alagbe EE, Abinusawa AP, John AN, Taiwo SO, Bolade OP (2021) Moringa oleifera as natural coagulant in water treatment and production of antifungal soap. In: IOP conference series: earth and environ science, vol 655. IOP Publishing, p 012007. https://doi.org/10.1088/1755-1315/655/1/012007
Ohia C (2014) Larvicidal efficacy of aqueous extract of Moringa oleifera seeds on malaria vector, (Anopeheles gambiae) and its toxicity effects on Mosquito fish, (Poecilia reticulata) A Dissertation Submitted to the University of Ibadan in Partial Fulfilment of the Requirement for the Award of Masters of Public Health (Environmental Health). Department of Environmental Health Sciences, Faculty of Public Health, College of Medicine, University of Ibadan, Nigeria
National Research Council (2006) Lost crops of Africa: volume II: vegetables. National Academies Press, Washington
Iqbal S, Bhanger MI (2006) Effect of season and production location on antioxidant activity of Moringa oleifera leaves grown in Pakistan. J Food Compos Anal 19(6–7):544–551. https://doi.org/10.1016/j.jfca.2005.05.001
El-Sayed AA, Amr A, Kamel OM, El-Saidi MM, Abdelhamid AE (2020) Eco-friendly fabric modification based on AgNPs@ Moringa for mosquito repellent applications. Cell 27:8429–8442. https://doi.org/10.1007/s10570-020-03355-8
Ojewumi ME, Emetere ME, Olikeze F, Babatunde DE (2018) Alternative solvent ratios for Moringa oleifera seed oil extract. Int J Mech Eng Technol 9:295–307
Ojewumi ME, Oyekunle DT, Ekanem GP, Obanla OR, Owolabi OM (2019) Extraction of oil from selected plants using response surface methodology [RSM]. In: Journal of physics: conference series, vol 1378, no 4. IOP Publishing, p 042019. https://doi.org/10.1088/1742-6596/1378/4/042019.
Vieira RF, Grayer RJ, Paton A, Simon JE (2001) Genetic diversity of Ocimum gratissimum L. based on volatile oil constituents, flavonoids and RAPD markers. Biochem Syst Ecol 29:287–304
Zaku SG, Emmanuel S, Tukur AA, Kabir A (2015) Moringa oleifera: An underutilized tree in Nigeria with amazing versatility: a review. Afr J Food Sci 9:456–461. https://doi.org/10.5897/AJFS2015.1346
Nakamura CV, Ueda-Nakamura T, Bando E, Melo AF, Cortez DA, Dias Filho BP (1999) Antibacterial activity of Ocimum gratissimum L. essential oil. Mem Inst Oswaldo Cruz 94:675–678. https://doi.org/10.1590/S0074-02761999000500022
Awah FM (2010) Antioxidant activity, nitric oxide scavenging activity and phenolic contents of Ocimum gratissimum leaf extract. J Med Plants Res 4:2479–2487. https://doi.org/10.5897/JMPR10.407
Sharma M, Alexander A, Saraf S, Saraf S, Vishwakarma UK, Nakhate KT (2021) Mosquito repellent and larvicidal perspectives of weeds Lantana camara L. and Ocimum gratissimum L. found in central India. Biocatal Agric Biotechnol 34:102040. https://doi.org/10.1016/j.bcab.2021.102040
Adefolalu FS, Ogbadoyi EO, Ndams IS, Mann A (2015) Larvicidal activities of N-hexane fraction of Ocimum gratissimum leaf against mosquito larvae and its GC-Ms analysis of phytoconstituents. J Appl Life Sci Int 2:175–188. https://doi.org/10.9734/JALSI/2015/17099
Elekwa I, Ugbogu AE, Okereke SC, Okezie E (2017) A review of selected medicinal plants with potential health benefits in South-Eastern Nigeria. Int J Pharm Chem Sci 6:162–171
Oparaocha ET, Iwu I, Ahanaku JE (2010) Preliminary study on mosquito repellent and mosquitocidal activities of Ocimum gratissimum (L.) grown in eastern Nigeria. J Vector Borne Dis 47:45
Adebayo KO, Aderinboye RY, Sanwo KA, Oyewusi IK, Isah OA (2019) Growth performance and fecal worm egg count of West African dwarf goats fed diets containing varying levels of Ocimum gratissimum (Scent leaf). Livest Res Rural Dev. 31:8. http://www.lrrd.org/lrrd31/8/yomow31124.html
Adebayo KO, Aderinboye RY, Sanwo KA, Oyewusi IK, Isah OA (2019) Microbial population and blood parameters of West African dwarf goats fed scent leaf (Ocimum gratissimum) as additive. Niger J Anim Prod 46:225–235
Ansari RA, Amah AK (2021) Phytochemical analysis and hepatoprotective potential of aqueous leaf extract of Ocimum gratissimum (Scent leaf). J Pharm Phytochem 10:192–195
Kalita B, Somi BS, Sharma AK (2013) Plant essential oils as mosquito repellent—a review. Int J Res Dev Pharm Life Sci. 3:741–747
Olawunmi MO, Taiwo AA, Oluwayemisi OA (2020) Assessment of antidiabetic activity of combined ethanolic leaf extracts from four medicinal plants: Ocimum gratissimum, Carica papaya, Cymbopogon citratus and Moringa oleifera in dexamethasone induced diabetic wistar rats. Afr J Sci Nat 5:29–36
Ojewumi ME, Oyekunle DT, Emetere ME, Olanipekun OO (2019) Optimization of oil from Moringa oleifera seed using Soxhlet extraction method. Korean J Food Health Converg 5:11–25
Ojewumi ME, Obanla RO, Taiwo SO, John AN (2021) Phytochemical screening and microbial assessment of Moringa oleifera seed crude oil extract. Rasayan J Chem 14:1835–1844. https://doi.org/10.31788/RJC.2021.1436226
Sparkman OD, Penton Z, Kitson FG (2011) Gas chromatography and mass spectrometry: a practical guide, 2nd edn. Acad Press, Cambridge
Tekin K, Karagöz S, Bektaş S (2014) A review of hydrothermal biomass processing. Renew Sustain Energy Rev 40:673–687. https://doi.org/10.1016/j.rser.2014.07.216
Ojewumi ME, Obanla RO, Ekanem GP, Nsionu JU (2021) Phytochemicals and antimicrobial properties of neem (Azadirachta indica) seed oil extract. In: ICEBE, 2021 (in press)
Faizi S, Sumbul S, Versiani MA, Saleem R, Sana A, Siddiqui H (2014) GC/GCMS analysis of the petroleum ether and dichloromethane extracts of Moringa oleifera roots. Asian Pac J Trop Biomed 4:650–4. https://doi.org/10.12980/APJTB.4.201414B141
Ojewumi ME, Omoleye JA, Ajayi AA, Obanla OR (2021) Molecular compositions and morphological structures of fermented African locust bean seed (Parkia biglobosa). Lett Appl NanoBioSci 11:3111–3119. https://doi.org/10.33263/LIANBS111.31113119
Gabi B, Lawal AO, Shariff HB (2012) Mosquito repellent activity and phytochemical characterization of essential oils from Striga hermonthica, Hyptis spicigera, and Ocimum basilicum leaf extracts. Br J Pharmacol Toxicol 3:43–48
Keziah EA, Nukenine EN, Danga SP, Younoussa L, Esimone CO (2015) Creams formulated with Ocimum gratissimum L. and Lantana camara L. crude extracts and fractions as mosquito repellents against Aedes aegypti L. (Diptera: Culicidae). J Insect Sci 15:45. https://doi.org/10.1093/jisesa/iev025
Ojewumi ME, Oyekunle DT, Amaefule CV, Omoleye JA, Ogunbiyi AT (2019) Investigation into alternative energy sources from waste citrus peel (Orange): approach to environmental protection. In: International conference on engineering for sustainable world. Journal of physics: conference series, vol 1378. IOP Publishing, p 022066. https://doi.org/10.1088/1742-6596/1378/2/022066.
Remia KM, Logaswamy S, Shanmugapriyan R (2017) Efficacy of botanical repellents against Aedes aegypti. Int J Mosq Res 4:126–129
Ojewumi ME, Banjo MG, Oresegun MO, Ogunbiyi TA, Ayoola AA, Awolu OO, Ojewumi EO (2017) Analytical investigation of the extract of lemon grass leaves in repelling mosquito. Int J Pharm Sci Res 8:2048–2055. https://doi.org/10.13040/IJPSR.0975-8232.8(5).2048-55
da Silva MR, Ricci-Júnior E (2020) An approach to natural insect repellent formulations: from basic research to technological development. Acta Trop 212:105419. https://doi.org/10.1016/j.actatropica.2020.105419
Marcus AC, Nwineewii JD (2015) Studies on the crude extract of Moringa oleifera leaf for preliminary identification of some phytochemicals and organic functions. J Appl Chem 8:01–05. https://doi.org/10.9790/5736-081220105
Jain PK, Soni A, Jain P, Bhawsar J (2016) Phytochemical analysis of Mentha spicata plant extract using UV-VIS, FTIR and GC/MS technique. J Chem Pharm Res 8:1–6
Saliu BK, Usman LA, Sani A, Muhammad NO, Akolade JO (2011) Chemical composition and antibacterial (oral isolates) activity of leaf essential oil of Ocimum gratissimum L. grown in North central Nigeria. Int J Curr Res 33:022–028
Zhang JS, Zhao NN, Liu QZ, Liu ZL, Du SS, Zhou L, Deng ZW (2011) A repellent constituent of essential oil of Cymbopogon distans aerial parts against two stored-product insects. J Agric Food Chem 59:9910–9915. https://doi.org/10.1021/jf202266n
Nikšić HA, Durić K, Omeragić E, Nikšić HE, Muratović S, Bečić F (2018) Chemical characterization, antimicrobial and antioxidant properties of Mentha spicata L. (Lamiaceae) essential oil. Bull Chem Technol Bosnia Herzeg 50:43–48
Pang X, Feng YX, Qi XJ, Wang Y, Almaz B, Xi C, Du SS (2020) Toxicity and repellent activity of essential oil from Mentha piperita Linn. leaves and its major monoterpenoids against three stored product insects. Environ Sci Pollut Res 27:7618–7627. https://doi.org/10.1007/s1135-019-07081-y
Isman MB, Machial CM, Miresmailli S, Bainard LD (2007) Essential oil-based pesticides: new insights from old chemistry. Pestic Chem Crop Prot Public Health Environ Saf 6:201–209
Cardé RT, Gibson G (2010) Host finding by female mosquitoes: mechanisms of orientation to host odours and other cues. Olfaction Vector Host Interact 8:115–142
Webb C, Doggett S, Russell R (2016) A guide to mosquitoes of Australia. Csiro Publishing, Clayton
Kim DH, Kim SI, Chang KS, Ahn YJ (2002) Repellent activity of constituents identified in Foeniculum vulgare fruit against Aedes aegypti (Diptera: Culicidae). J Agric Food Chem 50:6993–6996. https://doi.org/10.1021/jf020504b
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Ojewumi, M.E., Obanla, O.R. & Atauba, D.M. A review on the efficacy of Ocimum gratissimum, Mentha spicata, and Moringa oleifera leaf extracts in repelling mosquito. Beni-Suef Univ J Basic Appl Sci 10, 87 (2021). https://doi.org/10.1186/s43088-021-00176-x